Thermo Scientific 0.5 M EDTA, pH 8.0 is an aqueous solution of 0.22 µm membrane-filtered ethylendiaminetetraacetic acid (EDTA), sodium salt. It's ideal for biochemistry or molecular biology applications that require a chelator for divalent cations. By forming complexes with metal ions, EDTA inhibits metal-dependent enzymatic reactions. EDTA complexes metal ions at a 1:1 molar ratio.
Nucleic acids are normally stored in TE buffer that contains 1 mM EDTA, which prevents degradation of nucleic acids by metal dependent nucleases.
- Termination of metal dependent enzymatic reactions
- Protection of DNA against metal dependent nucleases
|The absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
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