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MassRuler™ Express Reverse DNA Ladder Mix, ready-to-use

Kód produktu: SM1293 Kód výrobce: SM1293 Kód dodavatele: {87C25D6F-3E13-454A-A253-844A8D1A7C59} Výrobce: UAB Fermentas
na dotaz
1000
DNA Ladder mixes specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions.
Zobraz detailní popis

Detailní popis

Thermo Scientific MassRuler Express Forward and Reverse DNA Ladder mixes are specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions (see Table 1). In the forward versions, the mass of each DNA fragment is directly proportional to the fragment size whereas, in the reverse versions, the mass is inversely proportional to fragment's size.

The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.

Highlights

  • Accurate DNA sizing and quantification – DNA ladder fragments are quantified spectrophotometrically
  • Fast and reliable DNA quantification – short separation distances under various electrophoresis conditions
  • Sharp bands
  • Easy-to-remember fragment sizes and quantities
  • Ready-to-use – premixed with 6X MassRuler DNA Loading Dye for direct loading and room temperature storage
  • Supplied with loading dye for sample DNA
6X MassRuler DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol, and 60 mM EDTA.
Hazardous No
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage and Loading Buffer 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.
Storage Condition -20 C

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