MagJET RNA Kit

Uses magnetic bead technology to purify total RNA from cultured mammalian cells, tissues, bacteria, and yeast. Detailní informace

Cena s DPH 9 861,50 Kč
Cena bez DPH 8 150,00 Kč
 ks
Dostupnost Skladem
Kód produktu K2731

Detailní popis MagJET RNA Kit

Source Quantity RNA yield, µg
Jurkat 106 cells 10-11
HeLa 106 cells 20-30
COS-7 5 × 105 cells 15-17
Mouse heart 5 mg 4-5
Mouse heart 20 mg 18-20
Mouse liver 5 mg 37-45
Mouse liver 20 mg 140-150
Mouse spleen 5 mg 27-30
Mouse brain 5 mg 4-7
Mouse lung 5 mg 5-9
Mouse kidney 5 mg 12-14
Mouse muscle 5 mg 3-4
E.coli ~109 cells 28-30
Saccharomyces cerevisiae ~108 cells 27-30

The Thermo Scientific MagJET RNA Kit is designed for fast and efficient purification of total RNA from cultured mammalian cells, tissues, bacteria and yeast.

The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low sample throughput users.

The resulting high quality purified RNA is free of proteins, nucleases and other contaminants or inhibitors, can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR and other enzymatic reactions. See Table 1 for typical total RNA yields from various sources.

Highlights

  • Versatile – suitable for total RNA purification from variety of biological samples, including eukaryotic cells, animal tissues, yeast and bacteria
  • Efficient – high yields of high-integrity RNA
  • High quality – isolates intact total RNA, free of inhibitors and gDNA contamination
  • Flexible – easily adjustable for high throughput automation platforms and manual processing

Applications

Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:

  • RT-PCR
  • RT-qPCR
  • Northern blotting
  • Nuclease protection assay

Includes

  • Lysis Buffer
  • MagJET Magnetic Beads
  • DNase I (lyophilized)
  • 10X Reaction Buffer with MgCl2 for DNase I
  • DNase I Reconstitution Buffer(conc.)
  • Wash Buffer 1 (conc.)
  • Wash Buffer 2 (conc.)
Hazardous Yes
Quality Control The MagJET RNA Kit is qualified by isolating RNA from 5 mg of frozen mouse muscle or heart following the protocol outlined in the manual. The quality of isolated RNA is evaluated spectrophotometrically and by agarose gel electrophoresis. The purified RNA has an A260/A280 ratio of 2.0±0.2. The functional quality of purified RNA is evaluated by RT-PCR.
Shelf Life 24 Months
Shipping Condition Ambient
Storage Condition Ambient

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Novinky

Čerstvé zprávy z MRC-Holland

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