MagJET Plant RNA Kit
Purifies plant total RNA from a variety of plant species and tissues without phenol/chloroform extraction using magnetic bead technology amenable to high-throughput assays.
Detailní popis
| Plant | Tissue (50 mg) | RNA yield, µg |
|---|---|---|
| Arabidopsis thaliana | leaf | 18-20 |
| Nicotiana tabacum | leaf | 15-20 |
| Tomato | leaf | 21-52 |
| Barley seedlings | leaf | 25-32 |
| Wheat shoots | leaf | 25-36 |
| Spinach | leaf | 24-30 |
| Onion | leaf | 8-12 |
| Rice | leaf | 16-19 |
| Corn | leaf | 13-20 |
| seeds | 20-40 | |
| Canola | leaf | 11-19 |
| stalk | 22-28 | |
| seeds | 30 | |
| Soy | leaf | 30-40 |
| seeds | 44 | |
| Cucumber | fruit | 18 |
| Potato | stalk | 10 |
| Sunflower | stalk | 4-5 |
| seeds | 40 |
Thermo Scientific MagJET Plant RNA Kit is designed for automated high throughput or manual purification of total RNA from wide variety of plant species and tissue types.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low sample throughput users.
The resulting high quality purified RNA is free of proteins, nucleases and other contaminants or inhibitors, can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR and other enzymatic reactions. See Table 1 for typical total RNA yields from various sources.
Highlights
- Versatile – suitable for any plant type and part, including woody, polyphenol-rich and lignified samples
- High yields of pure total RNA
- High quality – isolates intact total RNA, free of inhibitors and gDNA contamination. Isolated RNA is suitable for long term storage at -20°C
- Flexible – easily adjustable for high throughput automation platforms and manual processing
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
- RT-PCR
- RT-qPCR
- Northern blotting
- Nuclease protection assay
Includes
- Lysis Buffer
- MagJET Magnetic Beads
- DNase I (lyophilized)
- 2X DNase Buffer
- Manganese Chloride Solution
- DNase I Reconstitution Buffer
- Rebinding Buffer
- Wash Buffer 1 (conc.)
- Wash Buffer 2 (conc.)
- Water, nucle
CategoryName Hazardous Yes Hazardous: No Quality Control The MagJET Plant RNA Kit is qualified by isolating of total RNA from 50 mg of plant tissue following the protocol outlined in the manual. The quality of isolated RNA is evaluated spectrophotometrically and by agarose gel electrophoresis. The purified RNA has an A260/A280 ratio of 2.0±0.2 and the RNA integrity number (RIN) of ≥7. Shelf Life 24 Months Shelf Life: Shipping Condition Ambient Shipping Condition: Shipping Information SpecificationName SpecificationValue Storage Condition Ambient Storage Condition:
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