MagJET Plant Genomic DNA Kit

Purifies plant genomic DNA from a variety of plant species and tissues without phenol/chloroform extraction using magnetic bead technology amenable to high-throughput assays. Detailní informace

Cena s DPH 7 247,90 Kč
Cena bez DPH 5 990,00 Kč
 ks
Dostupnost Skladem
Kód produktu K2761

Detailní popis MagJET Plant Genomic DNA Kit

The Thermo Scientific MagJET Plant Genomic DNA Kit is designed for automated high throughput or manual purification of genomic DNA from a wide variety of plant species. The kit exploits an efficient magnetic particle technology eliminating need for phenol/chloroform extraction or alcohol precipitation.

The MagJET Plant Genomic DNA Kit procedures are optimized for the isolation of genomic DNA from 10-100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample.

The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput.

The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors, and can be used in a wide range of downstream applications such as PCR, qPCR or other enzymatic reactions. See Table 1 for typical genomic DNA yields from various sources.

Highlights

  • Versatile – suitable for any plant type and part, including woody, polyphenol-rich and lignified samples
  • High gDNA yields compared to traditional CTAB method
  • High quality – isolates high integrity gDNA, free of inhibitors and RNA contamination
  • Flexible – easily adjustable for high throughput automation platforms and manual processing

Applications

Isolated genomic DNA is ideal for use in common molecular biology procedures, including:

  • PCR and qPCR
  • FastDigest and conventional restriction digestion 
  • Southern blotting

Includes

  • Lysis Buffer A
  • Lysis Buffer B
  • MagJET Magnetic Beads
  • RNase A
  • Precipitation Solution
  • Wash Buffer 1 (conc.)
  • Wash Buffer 2 (conc.)
  • Elution Buffer
CategoryName
Hazardous Yes
Hazardous: No
Quality Control The MagJET Plant Genomic DNA Kit is qualified by isolating genomic DNA from 100 mg of plant tissue following the protocols outlined in the manual. The quality of isolated DNA is evaluated spectrophotometrically and by agarose gel electrophoresis. The purified DNA has an A260/A280 ratio of 1.8±0.3. A single band of more than 30 kb is observed after agarose gel electrophoresis and ethidium bromide staining. The functional quality of purified DNA is evaluated by digestion with restriction endonucleases.
Shelf Life 36 Months
Shelf Life:
Shipping Condition Ambient
Shipping Condition:
Shipping Information
SpecificationName
SpecificationValue
Storage Condition Ambient
Storage Condition:

RSSRSS

Novinky

Letní novinky od společnosti MRC-Holland

31. srpen 2017
V laboratořích Biogen a MRC-Holland se pracuje i vyvíjí i přes léto. Proto vám teď můžeme přinést nejnovější informace.

Čerstvé zprávy z MRC-Holland

15. červen 2017
Seznamte se s novými manuály pro Coffalyser.Net a novou platformou od Thermo Fisher Scientific pro analýzu Sangerova sekvenování a analýzu fragmentů.

Archiv novinek

FastDirect restrikční enzymy

Moderní linie restrikční enzymy pro rychlé DNA stříhání. Jsou schopné rozstříhat DNA za 5-15 minut.

Navštivte naše biobary

Nakupujte v biobarech a získejte 5% slevu z cen v našem eshopu! Biobary naleznete v Praze, Brně a v Českých Budějovicích.

© 2017, BIOGEN PRAHA s.r.o. – všechna práva vyhrazena

Partneři | Jak nakupovat | Služby | Mapa stránek

eBRÁNA | B2C Brána |

Zobrazit mobilní verzi

Přeskočit na začátek stránky