High Fidelity PCR Enzyme Mix
Detailní popis
Thermo Scientific High Fidelity PCR Enzyme Mix is a unique blend of Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. The two enzymes synergistically generate longer PCR products with greater yield and fidelity than Taq DNA Polymerase alone. Thus, fidelity of the High Fidelity PCR Enzyme Mix exceeds more than four times the fidelity of Taq DNA Polymerase. In addition, the enzyme mix can generate long amplicons up to 20 kb with viral DNA templates and up to 10 kb with genomic DNA templates. Such long amplicons are often difficult to generate with a single DNA polymerase.
Highlights
- Four-times greater accuracy than Taq DNA polymerase
- Long amplification products:
- up to 20 kb with viral DNA as template
- up to 10 kb with genomic DNA as template
- High yields
- Robust
Applications
- High yield PCR
- Long range PCR
- RT-PCR
Includes
- High Fidelity PCR Enzyme Mix (5 U/µL)
- 10X High Fidelity PCR Buffer
- 10X High Fidelity PCR Buffer with 15 mM MgCl2
- 25 mM MgCl2
- Nuclease-free water
Note
High Fidelity PCR Enzyme Mix generates both blunt-ended and 3'-dA tailed PCR products. Blunting of PCR product prior to cloning is highly recommended.
Hazardous | No |
Quality Control | Generation of 20 kb and 9 kb amplicons with lambda DNA and human DNA as templates, respectively. |
Storage Condition | -20 C |
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