GeneJET™ Viral RNA/DNA Purification Kit

Detailní popis

The Thermo Scientific GeneJET Viral DNA and RNA Purification Kit is designed for rapid and efficient purification of high quality viral nucleic acids from various human and mammalian liquid samples, and is compatible with common sample preservatives like EDTA, citrate, and heparin.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column. Viral nucleic acids from lysed samples bind to the column membrane while impurities are effectively removed during subsequent washing and centrifugation steps. Ready-to-use nucleic acids are eluted from the column.

The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA and RNA can be directly used in PCR, qPCR, or other nucleic acid based assays.

Highlights

• Efficient purification of viral DNA and RNA from human and mammalian plasma, serum, blood, and cell-free samples
• Linear yields from a wide range of viral titers
• Tested with a variety of DNA and RNA viruses (HIV, HBV, HCV, HPV, CMV, EBV)
• Downstream compatibility with (RT)-PCR, (RT)-qPCR, and other nucleic acid based assays.

Applications

• Downstream use in qualitative and quantitative nucleic acid based assays, e.g. (RT)-PCR, (RT)-qPCR

Includes

• Column Preparation Liquid
• Lysis Solution
• Wash Buffer 1 (concentrated)
• Wash Buffer 2 (concentrated)
• Eluent
• Proteinase K 
• Carrier RNA
• Spin Columns preassembled with Wash Tubes
• Wash Tubes (2 mL)