Endonuclease V, T.maritima

Thermo Scientific Endonuclease V, T. maritima (Endo V) is a  3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine (see Figure 1).

Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3' to a lesion. 

Highlights

• Optimal activity at temperatures of 65 to 70°C

Applications

• High-throughput methods for mutation research (see​ References 3,4)
• Studies in mutagenesis and DNA repair
• Mismatch cleavage
• Genotyping

Note

Use of this enzyme in certain applications may be covered by patents and may require a license. 

When the enzyme is in excess, the primary nicked products experience a  second nicking event on the complementary strand, leading to a  double-stranded break. At low concentrations, however, Endonuclease V  first nicks a DNA strand at the lesions located closer to the 5'-end of DNA molecule. Single-stranded DNA is cleaved with much lower efficiency. Mg²⁺ or Mn²⁺ ions are required for enzyme activity (see References 1-3).