Aminoallyl-dUTP, 50 mM Solution

Detailní popis

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

Applications

• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension

  Formula	C12H17N3O14P3Na3
  General Characteristics	lambdamax = 240 nm, extinction coefficient = 11.9 x 103 M-1 x cm-1 (pH 7.0) lambdamax = 290 nm, extinction coefficient = 7.8 x 103 M-1 x cm-1 (pH 7.0)
  Hazardous	No
  Molecular weight	589.2 (acid form: 523.2)
  Quality Control	Functionally tested in cDNA synthesis with RevertAid H Minus Reverse Transcriptase Greater than 96% purity confirmed by HPLC Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities
  Storage Condition	-20 C