Maxima™ H Minus Double-Stranded cDNA Synthesis Kit

Detailní popis

The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient one-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Highlights

• Efficient synthesis of full length double-stranded cDNA
• Fast – procedure completed in less than 2 hours
• Convenient – premixed components
• Complete – includes all primers, controls and residual RNA removal reagents

Applications

• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction

Includes

• First Strand Enzyme Mix
• 4X First Strand Reaction Mix
• Second Strand Enzyme Mix
• 5X Second Strand Reaction Mix
• 0.5 M EDTA, pH U/µL
• Control RNA, 0.5 µg/µL
• RNase I, 10 U/µL
• Control RNA, 0.5 µg/µL
• Oligo(dT)18 Primer, 100 µM, 0.5 µg/µL
• Random Hexamer Primer, 100 µM, 0.2 µg/µL
• Water, nuclease-free .
• Detailed Protocol