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Genomic DNA Purification Kit

Kód produktu: K0512 Kód výrobce: K0512 Kód dodavatele: {A1689F70-14E9-4842-B601-F1493BE8F8F5} Výrobce: Life Technologies Czech Republic s.r.o.
4 967,05 Kč
4 105,00 Kč bez DPH
do týdne

Selective detergent-mediated DNA precipitation system for genomic DNA isolation from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues. .

Zobraz detailní popis

Detailní popis

The Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

  • Efficient – 2 to 10 µg of genomic DNA from 0.2 mL of blood
  • Fast – procedure takes approximately 20 minutes
  • Universal – purifies genomic DNA from various sources
  • Flexible – easy to scale up and down
  • Safe – does not involve phenol extraction

Applications

  • Purification of high-quality genomic DNA from:
    • Whole blood or serum
    • Cell culture
    • Plant tissues
    • Mammalian tissues
    • Epithelium samples
    • Bacteria

Includes

  • Lysis Solution
  • Precipitation Solution
  • NaCl Solution
  • Detailed Protocol
Hazardous No
Quality Control
  • The kit is functionally tested for purification of genomic DNA from 0.2 mL of human blood.
  • The typical yield of DNA is 2 to 10 µg.
  • The quality of the purified DNA is evaluated in restriction digestion and in PCR amplification of a single-copy gene.
Storage Condition Ambient

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