10X Buffer EcoRI

Detailní popis

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

The following enzymes require unique buffers for optimal digestion:

AarI
AjiI
BamHI
BfuI
Bpu10I
BseXI
Bsp143I
Cfr9I
Cfr10I
Eam1105I
Ecl136II
Eco52I
EcoRI
KpnI
Lsp1109I
PacI
PasI
Ppu21I
SacI
ScaI
SdaI
SduI
SgeI
TaqI

1X Composition

50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
100 mM NaCl
0.02% Triton X-100
0.1 mg/mL BSA

Hazardous No
Storage Condition -20 C