FastDigest® MreI

5'...C GC C G G C G...3'
3'...G C G G C CG C...5'

Detailní informace

Cena s DPH 1 422,96 Kč
Cena bez DPH 1 176,00 Kč
 20 rxns
Dostupnost Skladem
Kód produktu FD2024

Detailní popis FastDigest® MreI

Thermo Scientific FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

  • 100% activity of all FastDigest enzymes in the universal buffer
  • 100% buffer compatibility with downstream applications
  • Complete digestion in 5-15 minutes
  • Direct loading on gels
  • No star activity
  • 176 FastDigest enzymes available

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP analysis

Note

The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity refer to product specifications.

Compatible Ends FastDigest AgeI (BshTI), FastDigest AvaI (Eco88I), FastDigest BsrFI (Cfr10I), FastDigest Kpn2I, BshTI, BsaWI, Cfr9I, Cfr10I, Eco88I, Kpn2I, NgoMIV, SgrAI.
Formulation 1 μL of enzyme (1 FDU) cleaves 1 μg of linearized pJET1 DNA with inserted MreI recognition site in 5 minutes at 37°C in 1X FastDigest Buffer.
Hazardous No
Isoschizomers Search for commercial isoschizomers using REsearch.
Recommended Reaction Conditions

1X FastDigest buffer or 1X FastDigest Green buffer at 37°C.
1 µL of FastDigest MreI is formulated to digest up to:

  • 1 µg of linearized pJET1 DNA with inserted MreI recognition site in 5 minutes
  • 1 µg of plasmid DNA in 5 minutes
  • 0.2 µg of PCR product in 5 minutes
  • 1 µg of genomic DNA in 5 minutes or 5 µg of genomic DNA in 30 minutes
Storage Condition -20 C

Reaction conditions

Reaction temperature Digestion time with 1 µL of FastDigest enzyme, min bp from end of DNA required for complete digestion Thermal inactivation Incubation time without star activity, hours
Lambda, 1 µg/20 µL Plasmid DNA, 1 µg/20 µL PCR product, ~0.2 µg/30 µL Genomic DNA, 1 µg/10 µL
37°C no sites
(pJET1-MreI DNA/Eam1105I,
5 min)
5 5 5 3 80°C, 5 min 16

Methylation Effects

  • Dam: never overlaps - no effect.
  • Dcm: never overlaps - no effect.
  • CpG: completely overlaps - blocked.
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.
Methylation type Sequence Cleavage effect
CpG CGCCGGCG Blocked

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
0 0 0 0 0 0
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
0 0 0 0 0 0

New sites generated by ligation

Newly generated recognition sites resulting from ligation of protruding compatible DNA ends

Recognition Sequence Second Enzyme Enzymes recognizing newly generated recognition sequence
CG^CCGGCG
  • BsaWI* (W^CCGGA)
  • Kpn2I (BspEI)/FastDigest Kpn2I (T^CCGGA)
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • BsaWI* (W^CCGGT)
  • BshTI (AgeI)/FastDigest AgeI (BshTI) (A^CCGGT)
  • Cfr10I (BsrFI)/FastDigest BsrFI (Cfr10I)* (R^CCGGT)
  • Cfr10I (BsrFI)/FastDigest BsrFI (Cfr10I)
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • Cfr10I (BsrFI)/FastDigest BsrFI (Cfr10I)* (R^CCGGC)
  • NgoMIV (G^CCGGC)
  • Cac8I
  • Cfr10I (BsrFI)/FastDigest BsrFI (Cfr10I)
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • NgoMIV
  • PdiI (NaeI)/FastDigest NaeI (PdiI)
  • Cfr9I (XmaI) (C^CCGGG)
  • Eco88I (AvaI)/FastDigest AvaI (Eco88I)* (C^CCGGG)
  • BcnI (NciI)/FastDigest NciI (BcnI)
  • Bme1390I (ScrFI)/FastDigest ScrFI (Bme1390I)
  • BssKI
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • SgrAI* (CR^CCGGCG)
  • Cac8I
  • Cfr10I (BsrFI)/FastDigest BsrFI (Cfr10I)
  • HpaII/FastDigest HpaII
  • MreI (Sse232I)/FastDigest MreI
  • MspI (HpaII)/FastDigest MspI
  • NgoMIV
  • PdiI (NaeI)/FastDigest NaeI (PdiI)
  • SgrAI
  • SgrAI* (CR^CCGGTG)
  • Cfr10I (BsrFI)/FastDigest BsrFI (Cfr10I)
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • SgrAI

Newly generated recognition sites resulting from fill-in of 5'-overhang and self-ligation

Recognition Sequence Newly generated sequence after reaction Enzymes that cleave the newly generated sequence
CG^CCGGCG CGCCGGCCGGCG
  • Bsh1285I (BsiEI)/FastDigest BsiEI (Bsh1285I)
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • [Cac8I]
  • [Cfr10I (BsrFI)/FastDigest BsrFI (Cfr10I)]
  • EaeI
  • CviJI
  • Eco52I (EagI)/FastDigest EagI (Eco52I)
  • [HpaII/FastDigest HpaII]
  • [MspI (HpaII)/FastDigest MspI]
  • [NgoMIV]
  • [PdiI (NaeI)/FastDigest NaeI (PdiI)]

Obsah balení

20 rxns

RSSRSS

Novinky

Tekutá biopsie a sekvenování MagBio

20. červen 2018
Představujeme vám pomůcky pro tekutou biopsii i sekvenování od společnosti MagBio. Přečtěte si výhody našich produktů a zjistěte víc o jejich správném používání.

HighPrep PCR společnosti MAGBIO

7. červen 2018
Produkt slouží pro čištění pomocí magnetických kuliček pro tvorbu NGS knihoven a post PCR. Systém dokáže plně nahradit konkurenční produkty, a to za pouhý zlomek nákladů.

Archiv novinek

FastDirect restrikční enzymy

Moderní linie restrikční enzymy pro rychlé DNA stříhání. Jsou schopné rozstříhat DNA za 5-15 minut.

© 2018, BIOGEN PRAHA s.r.o. – všechna práva vyhrazena

Partneři | Jak nakupovat | Služby | Mapa stránek

eBRÁNA | B2C Brána |

Zobrazit mobilní verzi

Přeskočit na začátek stránky