Neutralization Solution (from GeneJET™ Plasmid Miniprep Kit)

The Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.

Highlights

• Efficient – high yields of up to 20 µg of high quality plasmid DNA
• Fast – procedure takes less than 14 minutes
• Convenient – no phenol/chloroform extraction or alcohol precipitation required
• Pure – purified DNA is immediately ready to use

Applications

• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
  • FastDigest or conventional restriction digestion
  • Automated fluorescent and radioactive sequencing
  • PCR
  • In vitro transcription
  • Transformation

Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing.

Components

• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Wash Solution (concentrated)
• RNase A Solution
• Elution Buffer
• Spin Columns
• Collection Tubes
• Detailed Protocol