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Micrococcal Nuclease

Kód produktu: EN0181 Kód dodavatele: {F005DA2E-A386-4448-AF2D-3FD615158B45} Výrobce: Life Technologies Czech Republic s.r.o.
2 190,10 Kč
1 810,00 Kč bez DPH
do týdne
8000 Units
Micrococcal Nuclease (S7 Nuclease) is an endo-exonuclease that digests single-stranded and double-stranded DNA and RNA.
Zobraz detailní popis

Detailní popis

Thermo Scientific Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates (see Figure 1 in Supporting Data). The enzyme's activity is strictly dependent on Ca2+.

Applications

  • Hydrolysis of nucleic acids in crude cell-free extracts (see Reference 1)
  • Sequencing of RNA (see​ Reference 2)
  • Studies of chromatin structure (see​ Reference 3)
  • A model for protein folding and for structure-function studies (see​ References 4, 5)
    CategoryName
    Concentration 300 U/µL
    Definition of Activity Unit
    • One unit of the enzyme releases 1.0 A260 unit of acid-soluble products in 30 min at 37°C.
    • Enzyme activity is assayed in the following mixture: 100 mM sodium-glycine (pH 8.6), 10 mM CaCl2, and 4.5 mM calf thymus DNA.
    Hazardous No
    Hazardous: No
    Inactivation Inactivated by the addition of EGTA or EDTA.
    Inhibition Inhibitors: metal chelators, deoxythymidine 3' 5'-diphosphate.
    Molecular Weight 16.9 kDa monomer
    Quality Control 95% purity confirmed by SDS-PAGE.
    Shelf Life:
    Shipping Condition:
    Shipping Information
    Source E. coli cells with a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal nuclease).
    SpecificationName
    SpecificationValue
    Storage Buffer The enzyme is supplied in: 20 mM Tris-HCl (pH 7.6), 50 mM NaCl, and 50% (v/v) glycerol.
    Storage Condition -20 C
    Storage Condition: -20 C

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