Micrococcal Nuclease

Detailní popis

Thermo Scientific Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates (see Figure 1 in Supporting Data). The enzyme's activity is strictly dependent on Ca²⁺.

Applications

• Hydrolysis of nucleic acids in crude cell-free extracts (see Reference 1)
• Sequencing of RNA (see​ Reference 2)
• Studies of chromatin structure (see​ Reference 3)
• A model for protein folding and for structure-function studies (see​ References 4, 5)

  CategoryName
  Concentration	300 U/µL
  Definition of Activity Unit	One unit of the enzyme releases 1.0 A260 unit of acid-soluble products in 30 min at 37°C. Enzyme activity is assayed in the following mixture: 100 mM sodium-glycine (pH 8.6), 10 mM CaCl2, and 4.5 mM calf thymus DNA.
  Hazardous	No
  Hazardous:	No
  Inactivation	Inactivated by the addition of EGTA or EDTA.
  Inhibition	Inhibitors: metal chelators, deoxythymidine 3' 5'-diphosphate.
  Molecular Weight	16.9 kDa monomer
  Quality Control	95% purity confirmed by SDS-PAGE.
  Shelf Life:
  Shipping Condition:
  Shipping Information
  Source	E. coli cells with a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal nuclease).
  SpecificationName
  SpecificationValue
  Storage Buffer	The enzyme is supplied in: 20 mM Tris-HCl (pH 7.6), 50 mM NaCl, and 50% (v/v) glycerol.
  Storage Condition	-20 C
  Storage Condition:	-20 C