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Glycogen, RNA grade

Kód produktu: R0551 Kód výrobce: R0551 Kód dodavatele: {C84BAF14-0212-4CF0-B952-0DA9228313D9} Výrobce: Life Technologies Czech Republic s.r.o.
3 182,30 Kč
2 630,00 Kč bez DPH
do týdne
Store at -20°C
Glycogen is an inert carrier that increases the recovery of nucleic acids during preciptation.
Zobraz detailní popis

Detailní popis

Thermo Scientific Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation. Glycogen is insoluble in ethanol and forms a precipitate that traps target nucleic acids. During centrifugation, a visible pellet is formed which greatly facilitates handling of the precipitated nucleic acids. Glycogen quantitatively precipitates nucleic acids from diluted solutions with a  higher efficiency than that of tRNA, linear polyacrylamide, or sonicated DNA (see References 1-4).

We offer two formulations of aqueous glycogen solutions that allow for fast and efficient DNA or RNA recovery:

Glycogen, RNA grade, can be used for both RNA and DNA precipitation (#R0551).

Glycogen, molecular biology grade, recommended for DNA precipitation (#R0561).

Highlights

  • Ideal for high recovery of oligonucleotides (> 8 bases) and low amounts of DNA/RNA (> 20 pg) from diluted solutions
  • Forms clearly visible pellets
  • Does not interfere with enzymatic reactions†
  • Does not interfere with gel electrophoresis of nucleic acids
  • Does not interfere with spectrophotometrical determination of DNA/RNA concentration, (A260 to 280 measurements)
  • Compatible with DNA transformation and in vitro transfection

Footnote

† – At a final concentration up to 8 µg/µL, Glycogen does not interfere with PCR, DNA sequencing, DNA digestion by endonucleases, ligation, cDNA synthesis, DNA labeling, in vitro transcription, or bacterial transformation. At a final concentration up to 0.4 µg/µL, Glycogen does not affect in vitro transfection of eukaryotic cells.

CategoryName
Hazardous No
Hazardous: No
Molecular Weight
  • Glycogen molecules are highly branched structures, which are composed of thousands of glucose molecules bonded to each other.
  • The molecular weight of the largest individual glycogen molecule appears to be 8 million containing about 50,000 glucose molecules.
Quality Control
  • Absence of endo-, exodeoxyribonucleases, ribonucleases, phosphatases, proteases, and nicking activity confirmed by appropriate quality tests.
  • Glycogen, molecular biology grade, is functionally tested for DNA precipitation.
  • Glycogen, RNA grade, is functionally tested for RNA precipitation.
Shelf Life:
Shipping Condition:
Shipping Information
Source Oysters
SpecificationName
SpecificationValue
Storage Condition -20 C
Storage Condition: -20 C

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