GeneJET™ Plasmid Maxiprep Kit

The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient – high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
• High concentration of DNA purified – > 400 ng/µL
• Convenient – spin column format, centrifuge or vacuum protocols
• Fast – procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
  • Transfection of robust cell lines
  • FastDigest restriction enzyme digestion
  • Automated fluorescent sequencing
  • PCR
  • Cloning
  • Transformation
  • In vitro transcription

Includes

• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Endotoxin Binding Reagent
• Wash Solution I (concentrated)
• Wash Solution II (concentrated)
• RNase A Solution
• Elution Buffer (10 mM Tris-HCl, pH 8.5)
• GeneJET Maxi Purification Columns pre-assembled with collection tubes (50 mL)
• Collection tubes (50 mL)