GeneJET™ Genomic DNA Purification Kit
Kód produktu: K0721
Kód výrobce: K0721
Kód dodavatele: {6BBA9272-3108-40B7-8442-38E5CD3A1145}
Výrobce:
Life Technologies Czech Republic s.r.o.
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Silica membrane-based genomic DNA purification from mammalian cell culture and tissue samples, whole blood, bacteria and yeast. Provided in a convenient spin-column format.
Detailní popis
The Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.
Highlights
- Universal – can be used for both cell and tissue samples from a wide range of sources
- Efficient – high yields of high molecular weight genomic DNA
- Fast – 20 minute procedure following cell lysis
- Convenient – spin columns are capped and assembled with collection tubes
Applications
- Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
- PCR and qPCR
- FastDigest and conventional restriction digestion
- Southern blotting
Includes
- Proteinase K Solution
- RNase A Solution
- Digestion Solution
- Lysis Solution
- Wash Buffer I (concentrated)
- Wash Buffer II (concentrated)
- Elution Buffer
- GeneJET Purification Columns (pre-assembled with collection tubes)
- Collection tubes
- Detailed Protocol
| Source | Quantity | Yield, µg |
|---|---|---|
| Mammalian blood | 200 µL | 4 to 6 |
| Mouse heart | 10 mg | 10 to 15 |
| Mouse tail | 0.5 cm | 8 to 10 |
| Rat liver | 10 mg | 10 to 20 |
| Rat spleen | 5 mg | 20 to 30 |
| Rat kidney | 10 mg | 25 to 30 |
| Rabbit ear | 20 mg | 5 to 10 |
| Bacillus pumilis cells | 2x109 cells | 10 to 15 |
| Escherichia coli cells | 2x109 cells | 10 to 15 |
| HeLa cells | 2x106 cells | 15 to 20 |
| Jurkat cells | 5x106 cells | 25 to 30 |
| Saccharomyces cerevisiae cells | 1x108 cells | 3 to 5 |
| CategoryName | |
| Hazardous | No |
| Hazardous: | No |
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| Storage Condition | Ambient |
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Obsah balení
50 preps
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