Aminoallyl-UTP, 50 mM Solution

Detailní popis

Thermo Scientific Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH.

Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten.

Applications

• Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription

  Formula	C12H17N3O15P3Na3
  General Characteristics	lambdamax = 240 nm, extinction coefficient = 11.9 x 103 M-1 x cm-1 (pH 7.0) lambdamax = 290 nm, extinction coefficient = 7.8 x 103 M-1 x cm-1 (pH 7.0)
  Hazardous	No
  Molecular weight	605.2 (acid form: 539.2)
  Quality Control	Functionally tested in in vitro transcription with T7 RNA Polymerase Greater than 96% purity confirmed by HPLC Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities
  Storage Condition	-20 C