Thermo Scientific Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH.
Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten.
- Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription
Formula C12H17N3O15P3Na3 General Characteristics lambdamax = 240 nm, extinction coefficient = 11.9 x 103 M-1 x cm-1 (pH 7.0)
lambdamax = 290 nm, extinction coefficient = 7.8 x 103 M-1 x cm-1 (pH 7.0)
Hazardous No Molecular weight 605.2 (acid form: 539.2) Quality Control
- Functionally tested in in vitro transcription with T7 RNA Polymerase
- Greater than 96% purity confirmed by HPLC
- Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities
Storage Condition -20 C
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