Standartní PCR

PCR and RT-PCR

In cloning applications, it is crucial to maintain accurate DNA sequences during amplification. Thermo Scientific Phusion High-Fidelity DNA Polymerases have extremely low error rates and set a gold standard for high-fidelity PCR.

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer).
  • Polymerase speed allows for short extension times (15-30 s/kb).
  • Robust performance, minimal optimization needed.
  • Increased product yields with minimal amounts of enzyme.

Reverse Transcriptases

Maxima Reverse Transcriptases have been developed through in vitro evolution of M-MuLV reverse transcriptase to yield dramatically improved thermostability, robustness and increased synthesis rate compared to wild-type M-MuLV RT.

Thermal Cyclers

Small and fast, the Thermo Scientific Piko Thermal Cycler delivers superior thermal performance in a compact package and enables PCR runs in as little as 15 minutes. For routine PCR, the Thermo Scientific Arktik Thermal Cycler is a reliable and easy-to-use instrument designed to meet everyday requirements in any PCR laboratory.

Consumables

Thermo Scientific PCR plastics are manufactured with industry-leading processes and extensive quality control to ensure maximum PCR performance. The UTW® (ultra-thin wall) PCR plastics allow shorter PCR run times by improving heat transfer.


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