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Ruby Hot Start Master (2x)

Kód produktu: PCR-167XL Kód výrobce: PCR-167XL Výrobce: Jena Bioscience GmbH
65 767,13 Kč
54 353,00 Kč bez DPH
do týdne
100ML

Ruby Hot Start Master (2x)

Zobraz detailní popis

Detailní popis

Master mix for highly sensitive and specific PCR

Ready-to-Use Mixes for PCR

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term storage (up to 3 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid

Concentration: 2x conc.

Description:
Crystal Hot Start Master is a 2 x conc. ready-to-use master mix with blocked polymerase activity at ambient temperature.
The master mix is recommended for routine PCR applications, high throughput PCR or genotyping and provides an improved specificity and sensitivity when amplifying low-copy-number targets, working with complex backgrounds or when prolonged room-temperature set-ups are unavoidable.
The thermal activation at the onset of the initial denaturation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
It contains all reagents required for PCR (except template and primer) in a well-balanced ratio to ensure high specificity and minimal by-product formation in almost all PCR applications without the need of additional optimization steps.
The mix guarantees robust and reliable amplification results with a minimum of pipetting steps, saves time and reduces the risk of contaminations.
The total PCR assay volume is freely adaptable to individual protocols or the requirements of automated pipetting systems.

Content:

Cat.No. Master Mix PCR-grade water Assays x 50 μl
PCR-167S 4 x 1.25 ml 6 ml 200
PCR-167L 20 x 1.25 ml 2 x 12 ml 1000
PCR-167XL 100 ml 100 ml 4000

 

 

2 x concentrated PCR master mix containing aptamer inhibited hot start Taq polymerase, nucleotides (dATP, dCTP, dGTP, dTTP), KCl, (NH4)2SO4, MgCl2, density reagent, enhancing and stabilizing additives.

Recommended PCR assay:
Before starting, vortex the master mix thoroughly to assure homogeneity.

component stock. conc. 20 μl
assay
50 μl
assay
final conc.
Crystal Hot Start Master 2x 10 μl 25μl 1x
Primer Mix
or each primer
10 μM each primer 0.4-0.8 μl 1-2 μl 200-400 nM each primer
Template/ sample DNA   < 10 ng < 20 ng  
PCR-grade water   fill up to 20 μl fill up to 50 μl  

 

 

Recommended cycling conditions:
Before cycling, vortex PCR tubes or plates to assure homogeneity and centrifuge briefly to remove bubbles.

Initial
denaturation
95 °C 2 min 1x
Denaturation
Annealing1)
Elongation2)
95 °C
50 - 68 °C
72 °C
10 - 20 sec
10 - 20 sec
20 sec - 4 min

25 - 30x


1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

 

BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.

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