Uses magnetic bead technology to purify total RNA from cultured mammalian cells, tissues, bacteria, and yeast.
|Source||Quantity||RNA yield, µg|
|COS-7||5 × 105 cells||15-17|
|Mouse heart||5 mg||4-5|
|Mouse heart||20 mg||18-20|
|Mouse liver||5 mg||37-45|
|Mouse liver||20 mg||140-150|
|Mouse spleen||5 mg||27-30|
|Mouse brain||5 mg||4-7|
|Mouse lung||5 mg||5-9|
|Mouse kidney||5 mg||12-14|
|Mouse muscle||5 mg||3-4|
|Saccharomyces cerevisiae||~108 cells||27-30|
The Thermo Scientific MagJET RNA Kit is designed for fast and efficient purification of total RNA from cultured mammalian cells, tissues, bacteria and yeast.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low sample throughput users.
The resulting high quality purified RNA is free of proteins, nucleases and other contaminants or inhibitors, can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR and other enzymatic reactions. See Table 1 for typical total RNA yields from various sources.
- Versatile – suitable for total RNA purification from variety of biological samples, including eukaryotic cells, animal tissues, yeast and bacteria
- Efficient – high yields of high-integrity RNA
- High quality – isolates intact total RNA, free of inhibitors and gDNA contamination
- Flexible – easily adjustable for high throughput automation platforms and manual processing
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
- Northern blotting
- Nuclease protection assay
- Lysis Buffer
- MagJET Magnetic Beads
- DNase I (lyophilized)
- 10X Reaction Buffer with MgCl2 for DNase I
- DNase I Reconstitution Buffer(conc.)
- Wash Buffer 1 (conc.)
- Wash Buffer 2 (conc.)
|Quality Control||The MagJET RNA Kit is qualified by isolating RNA from 5 mg of frozen mouse muscle or heart following the protocol outlined in the manual. The quality of isolated RNA is evaluated spectrophotometrically and by agarose gel electrophoresis. The purified RNA has an A260/A280 ratio of 2.0±0.2. The functional quality of purified RNA is evaluated by RT-PCR.|
|Shelf Life||24 Months|
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