DreamTaq Green DNA Polymerase
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Detailní popis
Thermo Scientific DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. DreamTaq DNA Polymerase is an enhanced Taq polymerase optimized for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.
The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. For applications requiring PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless 10X DreamTaq Buffer (#B65) or purifying the PCR product before analysis. The 10X DreamTaq Green Buffer is optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM.
DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.
Highlights
- Direct loading of PCR product on gel
- High yields and high sensitivity of PCR
- Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
- Robust amplification with minimal optimization
- Incorporates modified nucleotides, but does not incorporate dUTP
Applications
- Routine PCR amplification of DNA fragments up to 6 kb
- Genotyping
- High throughput PCR
Includes
DreamTaq Green DNA Polymerase:
- DreamTaq Green DNA Polymerase
- 10X DreamTaq Green Buffer (includes 20 mM MgCl2)
DreamTaq Green PCR Master Mix (2X):
- DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
- Nuclease-free water
| 10X DreamTaq Green Buffer | A proprietary formulation which, in addition to the PCR buffer components, includes a density reagent and two tracking dyes. The density reagent allows direct loading of PCR products on a gel. The blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel) are included for monitoring electrophoresis progress. The dyes have excitation peaks at 424 nm and 615 nm. The 10X DreamTaq Green Buffer contains KCl and (NH4)2SO4 at a ratio optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM. |
| Definition of Activity Unit |
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| Hazardous | No |
| Inactivation | Inactivated by phenol/chloroform extraction. |
| Inhibition | Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1). |
| Quality Control | The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functionally tested in PCR. |
| Storage Buffer | The enzyme is supplied in 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, stabilizing agent and 50% (v/v) glycerol. |
| Storage Condition | -20 C |
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