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S1 Nuclease

Kód produktu: EN0321 Kód výrobce: EN0321 Kód dodavatele: {2C4E1AF7-C5F3-4914-909B-B2553CB999A5} Výrobce: Life Technologies Czech Republic s.r.o.
1 507,66 Kč
1 246,00 Kč bez DPH
do týdne
10000 Unit
S1 Nuclease is an endonuclease which degrades single-stranded DNA and RNA.
Zobraz detailní popis

Detailní popis

Thermo Scientific S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides (see Figure 1 in Supporting Data). It is five times more active on DNA than on RNA (see Reference 1). S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.

The enzyme is a glycoprotein with carbohydrate content of 18%.

Applications

  • Removal of single-stranded overhangs of DNA fragments (see​ Reference 2)
  • S1 transcript mapping (see​ References 3, 4)
  • Cleavage of hairpin loops
  • Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease III (see​ Reference 5)

Note

S1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations (see​ Reference 6).

5X Reaction Buffer 200 mM sodium acetate (pH 4.5 at 25°C), 1.5 M NaCl, and 10 mM ZnSO4.
CategoryName
Concentration 100 U/µL
Definition of Activity Unit One unit of the enzyme produces 1 µg of acid soluble deoxyribonucleotides in 1 min at 37°C.
Hazardous No
Hazardous: No
Inactivation Inactivated by heating at 70°C for 10 min in the presence of EDTA
Inhibition Inhibitors: metal chelators, PPi, Pi, 5'-ribonucleotides, and deoxyribonucleotides
Molecular Weight 29 kDa monomer
Quality Control
  • The absence of contaminating double-stranded DNA specific nuclease activity confirmed by appropriate quality test.
  • Functionally tested for the generation of unidirectional deletions in DNA.
Shelf Life:
Shipping Condition:
Shipping Information
Source Aspergillus oryzae
SpecificationName
SpecificationValue
Storage Buffer The enzyme is supplied in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 0.1 mM ZnCl2, and 50% (v/v) glycerol.
Storage Condition -20 C
Storage Condition: -20 C

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