Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube Luminaris Color HiGreen High ROX qPCR Master Mix

Luminaris Color HiGreen High ROX qPCR Master Mix

Ready-to-use qPCR master mixes with premixed UDG optimized for SYBR Green chemistry using standard cycling protocol. Master mixes available with or without blue color. . Detailní informace

Cena s DPH 5 112,25 Kč
Cena bez DPH 4 225,00 Kč
 250 x 20 µ
Dostupnost Skladem
Kód produktu K0361

Detailní popis Luminaris Color HiGreen High ROX qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Excellent qPCR performance:
    • Specificity – Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
    • Sensitivity – single copy detection
    • Wide linear range – accurate quantification across 9 orders of magnitude
    • Reproducibility and convenience – ready-to-use 2X master mix
    • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols

Applications

  • Gene expression analysis
  • siRNA validation
  • Genotyping
  • Pathogen detection

Includes

  • Luminaris Color HiGreen qPCR Master Mixes include Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.
    • Luminaris Color HiGreen High ROX qPCR Master Mix (2X) and Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) contain different amount of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
    • Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
  • Luminaris HiGreen qPCR Master Mixes include Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized colorless PCR buffer; supplied with nuclease-free water.
    • Luminaris HiGreen High ROX qPCR Master Mix (2X) and Luminaris HiGreen Low ROX qPCR Master Mix (2X) contain different amount of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
    • Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).

Real-time PCR instrument compatibility chart

  HiGreen High ROX qPCR Master Mix HiGreen Low ROX qPCR Master Mix HiGreen Fluorescein qPCR Master Mix HiGreen qPCR Master Mix
With blue dye K0361, K0362, K0363, K0364 K0371, K0372, K0373, K0374 K0381, K0382, K0383, K0384 K0391, K0392, K0393, K0394
Colorless K0961, K0962, K0953, K0964 K0971, K0972, K0973, K0974 K0981, K0982, K0983, K0984 K0991, K0992, K0993, K0994
Thermo Scientific PikoReal 24 Real-Time PCR System, PikoReal 96 Real-Time PCR System       checkmark
Life Technologies 7000, 7300, 7700, 7900, 7900HT, StepOne Plus™, StepOne™ checkmark      
7500, 7500 Fast, ViiA™ 7, QuantStudio™ 12K   checkmark    
Bio-Rad CFX96™, CFX384™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™       checkmark
MyiQ™, iQ5™, iCycler® iQ     checkmark checkmark
Stratagene Mx4000®, Mx3005P®, Mx3000P®   checkmark    
Roche Diagnostics LightCycler®480, LightCycler® 1.5, LightCycler® 2.0       checkmark
QIAGEN Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene Q       checkmark
Eppendorf MasterCycler® ep realplex       checkmark
Cepheid SmartCycler® SmartCycler® II       checkmark
Techne PrimeQ       checkmark
Illumina Eco Real-Time PCR System       checkmark
Hazardous No
Shelf Life 24 Months
Shipping Condition Frozen Gel Packs
Storage Condition -20 C
Storage Notes Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.

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