Lambda Exonuclease

Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction. Detailní informace

Cena s DPH 2 349,82 Kč
Cena bez DPH 1 942,00 Kč
 1000 units
Dostupnost Skladem
Kód produktu EN0561

Detailní popis Lambda Exonuclease

Thermo Scientific Lambda Exonuclease is a highly processive 5'=>3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA (see​ References 1-2)

Highlights

  • Active in Thermo Scientific PCR buffers

Applications

  • Generating single-stranded PCR products for use in:
    • DNA sequencing (see​ Reference 3)
    • Analysis of DNA single-strand conformation polymorphism (SSCP) (see​ Reference 4)
    • Rolling circle amplification
     
  • Producing single-stranded DNA from double-stranded DNA fragments
  • Cloning of PCR products (see Reference 5)

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.

10X Reaction Buffer 670 mM glycine-KOH (pH 9.4), 25 mM MgCl2, 0.1% (v/v) Triton X-100
CategoryName
Concentration 10 U/µL
Definition of Activity Unit
  • One unit of the enzyme catalyzes the release of 10 nmol of acid soluble reaction products from double-stranded substrate in 30 min at 37ºC.
  • Enzyme activity is assayed in the following mixture: 67 mM glycine-KOH (pH 9.4), 2.5 mM MgCl2, 0.1% (v/v) Triton X-100 and 20 µg/mL sonicated E. coli [3H]-DNA.
Hazardous No
Hazardous: No
Inactivation Inactivated by heating at 80°C for 15 min.
Inhibition Inhibitors: salt at relatively low concentration (0.2 M KCl, 0.1 M NaCl), p-chloromercuribenzoate.
Molecular Weight 26 kDa monomer
Quality Control
  • The absence of endodeoxyribonucleases confirmed by appropriate quality test.
  • Functionally tested for generating single-stranded DNA from double-stranded PCR product.
Shelf Life:
Shipping Condition:
Shipping Information
Source E. coli cells with a cloned exo gene of phage lambda.
SpecificationName
SpecificationValue
Storage Buffer The enzyme is supplied in:
25 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 50 mM NaCl, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol
Storage Condition -20 C
Storage Condition: -20 C

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