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FastRuler™ Ultra Low Range DNA Ladder, ready-to-use

Kód produktu: SM1233 Kód výrobce: SM1233 Kód dodavatele: {B2ED69BF-DF2C-4F79-94B7-DF8BBAAF12FE} Výrobce: Life Technologies Czech Republic s.r.o.
2 873,75 Kč
2 375,00 Kč bez DPH
do týdne
FastRuler DNA Ladders are specifically designed for fast sizing and approximate quantification of dsDNA in high throughput gels and conventional agarose gels.
Zobraz detailní popis

Detailní popis

FastRuler Ultra Low Range DNA Ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

The ladder is especially useful for electrophoresis of PCR products.

FastRuler Ultra Low Range DNA Ladder, ready-to-use contains dephosphorylated DNA fragments and is ideal for forward 5-end labeling with T4 Polynucleotide Kinase (see the Certificate of Analysis for the detailed labeling protocol).

Highlights

  • Fast separation (8 to 14 min)
  • Short separation distance (10 to 20 mm)
  • Sharp bands, easy-to-remember fragment sizes and quantities
  • Ready-to-use – premixed with loading dye
  • Supplied with loading dye solution for sample DNA
6× Orange DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
Bands
  • 200 bp
  • 100 bp
  • 50 bp
  • 20 bp
  • 10 bp
Hazardous No
Quality Control
  • Well-defined bands are formed during 8 to 14 min agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage and Loading Buffer 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol.
Storage Condition -20 C

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