FastAP™ Thermosensitive Alkaline Phosphatase

Thermosensitive Alkaline Phosphatase catalyzes the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins. Detailní informace

Cena s DPH € 75.61
Cena bez DPH € 62.49
 1000 units
Dostupnost Skladem
Kód produktu EF0651

Detailní popis FastAP™ Thermosensitive Alkaline Phosphatase

Thermo Scientific FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.

FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 minutes at 37°C. The enzyme is inactivated in 5 minutes at 75°C (see Figure 1 in Supporting Data). Therefore, removal of alkaline phosphatase is not required prior to ligation.

Highlights

  • Recombinant enzyme
  • Fast dephosphorylation – 10 minutes at 37°C
  • Fast and complete inactivation – 5 minutes at 75°C
  • Simultaneous digestion and dephosphorylation of vector DNA
  • 100% active in restriction enzyme and PCR buffers
  • One protocol for all types of DNA ends:
    • 5’-overhangs
    • 3’-overhangs
    • blunt-ends
    • single nucleotides
  • PCR clean-up in conjunction with Exo I
  • Protein dephosphorylation

Applications

  • Dephosphorylation of cloning vector DNA to prevent recircularization during ligation
  • Simultaneous digestion and dephosphorylation of vector DNA
  • PCR product clean-up: nucleotide degradation prior to sequencing of PCR product
  • Dephosphorylation of nucleic acid 5'-termini prior to labeling with T4 Polynucleotide Kinase
  • Other applications where dephosphorylation of DNA and RNA substrates is necessary
  • Protein dephosphorylation

Note

  • Binding of FastAP Thermosensitive Alkaline Phosphatase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 65°C for 10 minutes, and chill on ice prior to electrophoresis.
  • FastAP Thermosensitive Alkaline Phosphatase is active in all restriction enzyme buffers and may be added directly to digested DNA. Heat inactivation of the restriction enzyme before dephosphorylation reaction is not necessary.
10X FastAP Buffer 100 mM Tris-HCl (pH 8.0 at 37°C),50 mM MgCl2, 1 M KCl, 0.2% TritonX-100, and 1 mg/mL BSA.
Definition of Activity Unit One unit is amount of the enzyme required to dephosphorylate 1 µg of linearized pUC57 DNA5'-termini in 10 min at 37°C in FastAP buffer.
Hazardous No
Inactivation Inactivated by heating at 75°C for 5 min.
Inhibition Inhibitors: metal chelators.
Quality Control
  • Absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
  • Functionally tested for dephosphorylation of 5'-termini (overhanging, recessed, blunt) of DNA.
Source E. coli cells with a cloned bacterial AP gene.
Storage Buffer The enzyme is supplied in: 20 mM HEPES-NaOH(pH 7.4), 1 mM MgCl2, 0.1 mMZnCl2, 0.1% Triton X-100, and 50% (v/v) glycerol.
Storage Condition -20 C

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