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EheI (SfoI)

Kód produktu: ER0441 Kód výrobce: ER0441 Kód dodavatele: {5F454223-634A-45D0-9BE8-9FCF8E6CCE36} Výrobce: Life Technologies Czech Republic s.r.o.
3 581,60 Kč
2 960,00 Kč bez DPH
do týdne
500 Units

5'...G G CG C C...3'
3'...C C GC G G...5'

Zobraz detailní popis

Detailní popis

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

Unlike NarI, EheI completely digests lambda and pBR322 DNAs. Low salt, high glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity.

For methylation sensitivity refer to product specifications.

Conditions for 100% Activity
  • 1X Buffer Tango: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, and 0.1 mg/mL BSA
  • Incubate at 37°C
Digestion of Agarose-embedded DNA Minimum 20 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Double Digestion Perform double digestion using DoubleDigest.
Hazardous No
Isoschizomers Search for commercial isoschizomers using REsearch.
Storage Buffer EheI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.
Storage Condition -20 C

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X
G (green)
1X
O (orange)
1X
R (red)
1X
Tango
(yellow)
1X / 2X
Tango 37°C 20-50 50-100 0-20 0-20 100 20-50 1X or 2X

Methylation Effects

  • Dam: never overlaps - no effect.
  • Dcm: may overlap - no effect.
  • CpG: completely overlaps - blocked.
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.
Methylation type Sequence Cleavage effect
Dcm(CCWGG) 5'...Cm5CW GGCGCm5CW GG...3'
3'...G GWm5CCGCG GWm5CC...5'
No effect
CpG GGCGCC Blocked

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
20-50 50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
1 2 1 4 1 1
pTZ19R/U pTZ57R pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
0 0 0 0 0 4

New sites generated by ligation

Newly generated recognition sites resulting from ligation of blunt DNA ends

Recognition sequence Second restriction enzyme Restriction enzymes cleaving the newly generated recognition sequence
GGC^GCC
  • AanI (PsiI)/FastDigest PsiI (AanI) (TTA^TAA)
  • Bst1107I (BstZ17I)/FastDigest BstZ17I (Bst1107I) (GTA^TAC)
  • DpnI/FastDigest DpnI (GA^TC)
  • FaiI* (YA^TA)
  • FaiI* (YA^TG)
  • CviJI
  • AjiI (BmgBI)* (CAC^GTC)
  • ZraI (GAC^GTC)
  • Hin1I (BsaHI)/FastDigest BsaHI (Hin1I)
  • AluI/FastDigest AluI (AG^CT)
  • Bsh1236I (BstUI)/FastDigest Bsh1236I (CG^CG)
  • Bsp68I (NruI)/FastDigest NruI (RruI) (TCG^CGA)
  • CviJI* (RG^CT)
  • HpyCH4V (TG^CA)
  • MspA1I* (CMG^CTG)
  • PvuII/FastDigest PvuII (CAG^CTG)
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • CviJI
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI) (GG^CC)
  • CviJI* (RG^CC)
  • Eco147I (StuI)/FastDigest StuI (Eco147I) (AGG^CCT)
  • MlsI (MscI)/FastDigest MscI (MlsI) (TGG^CCA)
  • BmgT120I
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • Cfr13I (Sau96I)/FastDigest Sau96I (Cfr13I)
  • CviJI
  • Ecl136II (EcoICRI)/FastDigest Ecl136II (GAG^CTC)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (CCG^CTC)
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • CviJI
  • MnlI/FastDigest MnlI
  • Eco32I (EcoRV)/FastDigest EcoRV (Eco32I) (GAT^ATC)
  • LweI (SfaNI)/FastDigest SfaNI (BmsI)
  • Eco47III (AfeI)/FastDigest AfeI (Eco47III) (AGC^GCT)
  • FastDigest HaeII (BfoI)
  • HhaI/FastDigest HhaI
  • Hin6I (HinP1I)/FastDigest HinP1I (Hin6I)
  • FspAI/FastDigest FspAI* (RTGC^GCAC)
  • FspAI/FastDigest FspAI* (RTGC^GCAT)
  • NsbI (FspI)/FastDigest FspI (NsbI) (TGC^GCA)
  • HhaI/FastDigest HhaI
  • Hin6I (HinP1I)/FastDigest HinP1I (Hin6I)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (GAG^CGG)
  • MspA1I* (CMG^CGG)
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • CviJI
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • PdiI (NaeI)/FastDigest NaeI (PdiI) (GCC^GGC)
  • SatI (Fnu4HI)/FastDigest Fnu4HI (SatI)
  • TauI/FastDigest TauI
  • SrfI (GCCC^GGGC)
  • Cac8I

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